BME 4051L Biomedical Engineering Laboratory 2
Fridays
|
Instructor: James Byrne Office:
EAS 2612 Phone 348-1409 Email:
james.byrne@fiu.edu
Textbook:
Handouts will be provided
Course Description:
BME Lab II: Focus on Transport and Instrumentation
Course
Objectives: By the
end of this course students should be able to 1.
Design and conduct experiments involving transport,
mechanics and instrumentation. 2.
Apply multivariable calculus and
differential equations to the design and execution of experiments. 3.
Develop and demonstrate an ability to make measurements on
and interpret data from living systems. 4.
Develop skill in the use of modern engineering
tools 5.
Analyze and interpret data. 6.
Address problems associated with the interaction between
living and non-living materials and systems. 7.
Utilize analytical and experimental methods and modern
computer technology for decision-making
and to solve realistic biomedical engineering problems. 8.
Work effectively with others in a team while
simultaneously maintaining independent and creative thought. 9.
Communicate effectively and to articulate technical
matters using verbal, written, and graphic
techniques. Points
Distribution: Report Grading Criteria: Technical Content 60% Figures, Equations, Tables 30% Spelling and Grammar 5% Style (impress me points) 5% Course Grading Criteria: Experimental Summary Reports 70%
Short Engineering
Report 30% Attendance is mandatory
and will be taken in writing before class begins. Late arrival will mean 5%
will be deducted from your report. Lab reports must be turned in two weeks
following the experiment. Software assignments may be turned in as prints
outs and text files. Late lab reports
will not be accepted. Grading is done on an
equally weighted line, D- gets 1 point, and A+ gets 12 points. Averages are to the
nearest point, rounding is 1.5 rounds to 1, 1.51 rounds to 2. An example
grading worksheet will be issued to the class. Tentative Course Outline: ___________________________________________________________________________________ Completion of FIU’s online laboratory safety
training will be required of all students. The courses completed shall be: 1.
1001 Hazard
Communication 2.
1003 Bloodborne
Pathogens Exposure Control 3.
1004 Laboratory
Safety 4.
1008 FIU
Hazardous Waste Management Training 5.
1013 Research
Involving Animals All students are required to earn a certificate in
each if these courses. Failure to earn
any required certificate will result in a reduction in grade by one grade
level. Students already holding certificates will not be required to
re-certify. Five
laboratory exercises will be performed: Medical Instrumentation and Signal Processing·
Biopac
EMG Lab ·
Standard
and Integrated EMG ·
Motor unit recruitment and fatigue. ·
Signal
processing of EMG signals, power spectrum. This laboratory provides experience with
signal processing and instrumentation with living subjects and addresses all
of the course objectives. Preparative Column Chromatorgraphy
· Lysing whole blood. · Determination of void volume. · Purifying
Hemaglobin ·
Measuring the
Spectrum of the extracted HgB These experiments
provide experience with transport modeling and fluid mechanics theory,
instrumentation and preparative techniques. Course Objectives 1, 2, 4, 5, 7,
8, 9. are met
Radioisotope Laboratory· Use of Gamma Spectrometer. ·
Measure
laboratory background radiation, ·
Cs 137
calibration source, Counting statistics ·
Half life
determination ·
Inverse square
law. ·
Measure mass
attenuation though different foils. ·
Determine
attenuation coefficients. Course
Objectives 1, 2, 4, 5, 8, 9 are met in
this lab. Calcium metabolism of murine C2/C12 myocytes·
Culture
of C2/C12 cells is provided in Ca ++ free media. ·
Calcium
stain Fura is applied. ·
Dual color fluorescent imaging of cell culture
·
Ratio Images to obtain Bound /Unbound Calcium
Ratio ·
Apply Ca++ ion containing buffer
and repeat images ·
Prepare Standard Curve to quantify the ratio
images. ·
The students will learn fluorescent microscopy
instrumentation fundamentals, use
image processing software and study ion transport in living cells. All Course Objectives are addressed in this
lab. Isolated Perfused Rat Heart· Proper and ethical use of animals in research. · Measure the effects of pharmacological agents on the hemodynamics of the isolated rat heart · membrane oxygenators · measure pressures using fluid filled and catheter tip pressure transducers · measure flow using an in-line transducers · Electrophysiology may also be included since the heart can be artificially paced. ·
Experiments on isolated preps to compare to
experiments on intact animals All
Course Objectives are addressed in this lab. Gas Transport through membranes in the presence of Haemogobin· Passive Transport of gases through membranes · Oxygen capacity of fluids with and without haemoglobin · Measure PO2 · Measure flow. · Couple Navier Stokes and diffusion through a membrane to model the system ·
Use
different tubing dimensions to demonstrate the effects of changing the wetted
area All Course Objectives are addressed in this lab. BME 4051 L Class Calendar
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|