TA: TBA, TEC 3477, 348-6248
EMAIL:
Class: MW
Room: EC 1105
OFFICE HOURS: M-Th
TEXTBOOK: "Tissue Engineering" 1st Ed., Bernhard O. Palsson, Jeffrey A. Hubbell, Robert Plonsey and Joseph D. Bronzino, CRC Press, 2003
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SUPPLEMENTS: “Culture of Animal Cells: A Manual of Basic Technique,” R. Ian Freshney, Wiley-Liss, 2000; " Principles of Tissue Engineering," Eds. Robert P. Lanza, Robert Langer, Joseph Vacanti Academic Press, 2000.
PREREQUISITES: BME 3992, BME 4990
COURSE DESCRIPTION: The objective of this course is to gain an appreciation for the concepts and applications of molecular, cellular, and tissue engineering via lectures, case studies and laboratory practice. Basic techniques in biotechnology, cell and tissue dynamics, stem cell technology, and cell transplantation are overviewed. Physiology of cell growth and culturing along with adhesion dynamics will be taught.
COURSE OBJECTIVES: Overview of tissue engineering theory and principles with emphasis on cell behavior and morphology. Concepts and theories in engineering tissue replacements are introduced. Lecture and laboratory practicum in cell growth and development, characterization, and functionality are administered in an application format. This course introduces molecular, cellular, and tissue engineering in a hands-on type of environment. Students will learn and employ cell isolation, maintenance and characterization of cell populations.
BME 4332: The Final Project will be a 8-10 page summary on cell culture growth and analysis in the course.
GRADE
DISTRBUTION: Class participation/Quizzes 10%
Exam 1 25%
Exam 2 25%
Final (
Final
Project (
Exams will
be one hour in duration. All exams will be comprised of multiple-choice and
essay-based questions. There will be no
makeup exams without Chairman’s written approval.
Grading
Scale:
A³90, 90 >A- ³ 86.7, 86.7 > B+ ³ 83.3, 83.3 > B ³ 80, 80 > B- ³ 76.7, 76.7 > C+ ³ 73.3, 73.3 > C ³ 70.0,
70.0 > C- ³ 66.7, 66.7 > D+ ³ 63.3, 63.3 > D ³ 60, 60 > D- ³ 57.7, 57.7 > F
Laboratory
Practicum
Students will not be allowed to begin the experiments until the EHS certifications are submitted. In the event that the certification is incomplete or late, 25% grade reduction (Final Grade) will be enforced. There will be no makeup on missed experiments and no continuation if you any missed. Attendance will be taken.
Experiment 1: Animal cell culture techniques
Experiment 2: Cell adhesion/spreading on natural/synthetic scaffolds and effect of CAMs
Times and dates for experiments are to be determined in class. In additional, a small culture kit needs to be purchased. Details to follow.
*Written reports of the cases should be no more than 2 pages (single space, 12 pt. Font 1-inch margins).
Please
be concise and to the point.
Educational Objectives for Cell and Tissue Engineering (BME 4332)
The objective of this course is to provide an introduction to the overview of tissue engineering theory and principles with emphasis on cell behavior and morphology. Concepts and theories in engineering tissue replacements are introduced. This course introduces the application of cellular engineering in a hands-on type of environment. Lecture and laboratory practicum in cell growth and development, characterization, and functionality are learned. Students will be taught and employ cell isolation, maintenance and characterize of cell populations. Structure-property relationships in tissue-biomaterial will be explored along with analytical tools used to evaluate their properties. Specific objectives and expected outcomes are list below:
1. Biology of cultured cells.
Students should be able to:
A. Understand the historical background and general use of cells as therapeutic agents
B. Be certified and adept at aseptic techniques in culturing cells, handling and disposal of waste, and laboratory safety.
C. Understand cell growth and rates
D. Understand general biological (cell-cell), mechanical (cell-ECM) and surface (cell-biomaterial) properties of cell interactions.
E. Be able to employ techniques to primary cell culturing.
2. Develop and apply common biomaterials in in vitro tissue design
Students should be able to:
A. Understand the use of various biomaterials in formulation of cell therapies
B. Understand the effect of materials on cell morphology and tissue formation
3. Apply analytical techniques in cell/tissue characterization
Students should be able to:
A. Identify various cellular processes (cell differentiation; e.g. myoblasts → myotubules)
B. Identify and employ various techniques for cell/tissue processing (e.g. fixation, cryopreservation)
C. Identify biological response to materials and the host [e.g. viability (cell), necrosis (cell/tissue)]
4. Recognize Clinical Applicability
Students should be able to:
A. Design and implementation of engineered tissues.
B. Be able to understand tissue functionality and assessment.
C. Understand general types of tissue replacements or tissue engineered medical products (TEMPs).
D. Understand regulatory and ethical issues