Cellular and Tissue Engineering: Theory and Methodology

 BME 6993: Spring 2004

 

 

INSTRUCTOR: Dr. E. Crumpler, TEC 2613, 348-1864

EMAIL:  crumpler@fiu.edu     

 

TA: Ms. Martha Vallejo, TEC 3477, 348-6248

EMAIL:  mvalle02@fiu.edu     

 

Class:              TTH   16:10 – 17:25

 

Room:                        EC 2410

     

                             

OFFICE HOURS:  T-Th 2:00-3:00, by Appt.

 

TEXTBOOK: "Tissue Engineering" 1st Ed., Bernhard O. Palsson and Sangeeta N. Bhatia, Pearson Pentice Hall, 2003

     .

SUPPLEMENTS:   “Culture of Animal Cells: A Manual of Basic Technique,” R. Ian Freshney, Wiley-Liss, 2000; " Principles of Tissue Engineering," Eds. Robert P. Lanza, Robert Langer, Joseph Vacanti Academic Press, 2000.

 

PREREQUISITES:  BME 3992, BME 4990

 

COURSE DESCRIPTION:  The objective of this course is to gain an appreciation for the concepts and applications of tissue engineering via lectures, case studies and laboratory practice. Basic techniques in biotechnology, cell and tissue dynamics, stem cell technology, and cell transplantation.  Physiology of cell growth and culturing along with adhesion dynamics will be taught.

 

COURSE OBJECTIVES:  Overview of tissue engineering theory and principles with emphasis on cell behavior and morphology.  Concepts and theories in engineering tissue replacements are introduced.  Lecture and laboratory practicum in cell growth and development, characterization, and functionality are administered in an application format. This course introduces molecular, cellular, and tissue engineering in a hands-on type of environment.  Students will learn and employ cell isolation, maintenance and characterize of cell populations.

 

BME 4332: The Final project will be a 8-10 page summary on cell culture growth and analysis in the course.

 

 

GRADE DISTRBUTION:        Class participation/Quizzes                    10%

Exam 1                                                 15%

Exam 2                                                 15%

Final                                                     20%

Case study/Lab reports                         20%

                                                Final Project                                         20%

 

      Exams will be one hour in duration. All exams will be comprised of multiple-choice and essay-based questions.  There will be no makeup exams without Chairman’s written approval.

 

Grading Scale:

 

A³90, 90 >A- ³ 86.7, 86.7 > B+ ³ 83.3, 83.3 > B ³ 80, 80 > B- ³ 76.7, 76.7 > C+ ³ 73.3, 73.3 > C ³ 70.0,

70.0 > C- ³ 66.7, 66.7 > D+ ³ 63.3, 63.3 > D ³ 60, 60 > D- ³ 57.7, 57.7 > F

 

 

 

 

 

 

Lectures, case based studies and Labs:

 

Week 1 (1/6) Lecture 1,2: Introduction to tissue engineering and the

case based method (assignment of case 1); EHS Certification (All certificates) due by Week 3.

 

Week 2 (1/13) Lecture 3,4: Cell/extracellular matrix interactions (discussion of case 1,

written report* for case 1 due 1/20; assignment of case 2); Cell culture techniques

 

Week 3 (1/20) Lecture 5,6: The cellular processes and interactions with synthetic materials

(discussion of case 2, written report for case 2 due 1/27; assignment of

case 3); Experiment 1- Cell culture (Quiz 1)

 

Week 4 (1/27) Lecture 7,8: Transport of nutrients and metabolites (vascularization)

(discussion of case 3, written report for case 3 due 2/3; assignment of case 4)    

Exam I (January 29, 2004)

 

Week 5 (2/3) Lecture 9,10: Polymer scaffolds for tissue engineering (discussion of case 4,

written report for case 4 due 2/10; assignment of case 5)

 

Week 6 (2/10) Lecture 11,12: The tissue microenvironment and bioreactor design (discussion

of case 5, written report for case 5 due 2/17; assignment of case 6); Experiment 2 – Scaffolding (Q2)

 

Week 7 (2/17) Lecture 13,14: Tissue Engineering of Skin

 

Week 8 (2/24) Lecture 15,16: Tissue Engineering of Cartilage

                            Exam II (February 26, 2004)

 

Week 9 (3/2) Lecture 17,18: Tissue Engineering of Bone

 

Week 10 (3/9) Lecture 19,20: Tissue Engineering of Muscle

    (Conference – Ms. Vallejo); Experiment 3 - 1º isolation (Q3)

 

Week 11 (3/16) Lecture 21,22: Tissue Engineering of Nerve

 

Spring Break (22 – 27 March)

 

Week 12 (3/30) Lecture 23,24: Tissue Engineering of Pancreas; Experiment 4 - Characterization

                                 Exam III (April 1, 2004)

Week 13 (4/6) Lecture 25,26: Tissue Engineering of Liver (Q4)

 

Week 14 (4/13) Lecture 27,28: Review

 

Final Exam – April , 2004

 

 

Laboratory Practicum

 

Students will not be allowed to begin the experiments until the EHS certifications are submitted.  In the event that the certification is incomplete or late, 25% grade reduction (Final Grade) will be enforced. There will be no makeup on missed experiments and no continuation if you any missed.  Attendance will be taken.

 

Experiment 1: Animal cell culture techniques

 

Experiment 2: Cell adhesion/spreading on natural/synthetic scaffolds and effect of CAM

 

Experiment 3:  1º Skeletal fibroblast cell isolation.

 

Experiment 4: Assessment of implant characterization, cell, tissue/organ fixation

 

Times and dates for experiments are to be determined in class. In additional, a small culture kit needs to be purchased.  Details to follow.

 

*Written reports of the cases should be no more than 2 pages (single space, 12 pt. Font 1-inch margins).

Please be concise and to the point.

 

 

 

Educational Objectives for Cell and Tissue Engineering (BME 4332)

 

The objective of this course is to provide an introduction to the overview of tissue engineering theory and principles with emphasis on cell behavior and morphology.  Concepts and theories in engineering tissue replacements are introduced. This course introduces the application of cellular engineering in a hands-on type of environment.  Lecture and laboratory practicum in cell growth and development, characterization, and functionality are learned. Students will be taught and employ cell isolation, maintenance and characterize of cell populations.  Structure-property relationships in tissue-biomaterial will be explored along with analytical tools used to evaluate their properties.  Specific objectives and expected outcomes are list below:

 

 

1.                  Biology of cultured cells.

 

Students should be able to:

 

A.     Understand the historical background and general use of cells as therapeutic agents

B.      Be certified and adept at aseptic techniques in culturing cells, handling and disposal of waste, and laboratory safety.

C.     Understand cell growth and rates

D.     Understand general biological (cell-cell), mechanical (cell-ECM) and surface (cell-biomaterial) properties of cell interactions.

E.      Be able to employ techniques to primary cell culturing.

 

2.                  Develop an understanding and application of common biomaterials in in vitro tissue design

 

Students should be able to:

 

A.     Understand the use of various biomaterials in formulation of cell therapies

B.     Understand the effect of materials on cell morphology and tissue formation

 

3.                  Develop an understanding of analytical techniques in cell/tissue characterization

 

Students should be able to:

 

A.     Identify various cellular processes (cell differentiation; e.g. myoblasts → myotubules)

B.     Identify and employ various techniques for cell/tissue processing (e.g. fixation, cryopreservation)

C.     Identify biological response to materials and the host [e.g. viability (cell), necrosis (cell/tissue)]

 

4.                  Recognition of Clinical Applicability

 

Students should be able to:

 

A.     Design and implementation of engineered tissues.

B.     Be able to understand tissue functionality and assessment.

C.     Understand general types of tissue replacements or tissue engineered medical products (TEMPs).

D.     Understand regulatory and ethical issues